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Image Search Results
Journal: Frontiers in Immunology
Article Title: Myeloid Vamp3 deletion attenuates CFA-induced inflammation and pain in mice via ameliorating macrophage infiltration and inflammatory cytokine production
doi: 10.3389/fimmu.2023.1239592
Figure Lengend Snippet: Myeloid Vamp3 deletion reduced inflammation and pain-related gene expression. (A-H) In comparison to WT mice, RT-qPCR showed the downregulation of IL-6 (A) , TNF-α (B) , CXCL11 (C) , TIMP-1 (D) , CD68 (E) , IL-1β (F) , COX-2 (G) , and CD54 (H) gene transcription levels in the ipsilateral plantar skins of Vamp3 Δmyel mice at day 14 after CFA injection. (I-K) CFA-injected paws of WT and Vamp3 Δmyel mice were dissected and homogenized in RIPA lysis and extraction buffer. The extracts were subjected to SDS-PAGE and Western Blotting. IL-1β (I) , COX-2 (J) , and CD54 (K) proteins were detected using their specific antibodies. As indicated, GAPDH or β-actin was probed as an internal loading control. (L) The ratios calculated for IL-1β, COX-2, and CD54 and the requisite internal standard (i.e. β-actin or GAPDH) for the CFA-injected paws of Vamp3 Δmyel mice were expressed relative to those for WT controls. Data are presented as mean ± SEM, n≥3 as indicated in the graph. *p<0.05, **p < 0.01, ***p < 0.001; unpaired two-tailed Student’s t-test, compared to the WT controls.
Article Snippet: The process was carried out in a similar manner, except for the use of specific antibodies:
Techniques: Gene Expression, Comparison, Quantitative RT-PCR, Injection, Lysis, Extraction, SDS Page, Western Blot, Control, Two Tailed Test